Journal: Scientific Reports

Article Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors

doi: 10.1038/srep14213

Figure Lengend Snippet: Data were normalized and the scale is indicated below the figures. ( a ) Unsupervised hierarchical clustering was performed for individual technical replicates. ( b ) Competitive inhibition of EV interactions with lectin microarray with mannose (Man) and N -acetylglucosamine (GlcNAc). ( c ) Lectin microarray of PNGase F-treated EVs. Significance (* = p ≤ 0.05) was determined by the ANOVA test.

Article Snippet: Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described .

Techniques: Inhibition, Microarray

Journal: bioRxiv

Article Title: A Photonic Biosensor-Integrated Tissue Chip Platform for Real-Time Sensing of Lung Epithelial Inflammatory Markers

doi: 10.1101/2022.06.21.497028

Figure Lengend Snippet: a) Design in GDS (Graphic Design System) format used to produce photolithographic masks for the photonic chip. The overall footprint is 5.8 × 4.0 mm, with 2 banks of 7 × 2 ring resonators, each with a total diameter of 300 μm including the trench (open area in the top layer of SiO 2 allowing direct contact between the ring resonator and analyte-containing solution). One temperature control ring (under oxide) is also provided in each bank. b) A photograph of the chip after fabrication and dicing. c) Functionalization chemistry using a succinic anhydride-bearing silane. d) Photomicrograph of a ring bank, with the antibody layout superimposed. e) An image acquired by the microarrayer immediately after spotting shows reproducible spotting of antibody solutions and StabilGuard over the rings. The dark spots are ∼250 pL drops settled in the ring trenches.

Article Snippet: After silanization, antibody solutions were printed on the rings using a Scienion SX SciFLEXarrayer piezoelectric microarrayer equipped with a PDC-60 capillary nozzle.

Techniques: Control

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease

doi: 10.3390/ijms25094625

Figure Lengend Snippet: Lectins used in microarray; their glycan specificity and reactivity with a PD transferrin (weak “+” and strong “++”).

Article Snippet: A microarray analysis was performed with each sample separately using biotinylated lectins, epoxy microarray slides (NEXTERION Slide E, Schott, Germany), a non-contact piezoelectric sciFLEXARRAYER S1 microarray spotter and a piezo dispense capillary PDC 80 (Scienion AG, Berlin, Germany) [ ].

Techniques: Microarray, Glycoproteomics, Whole Genome Amplification

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease

doi: 10.3390/ijms25094625

Figure Lengend Snippet: Reactivity of transferrin isolated from healthy persons (H) and patients on peritoneal dialysis (PD) with lectins. A statistically significant difference between groups ( p < 0.05) is labeled with “*”.

Article Snippet: A microarray analysis was performed with each sample separately using biotinylated lectins, epoxy microarray slides (NEXTERION Slide E, Schott, Germany), a non-contact piezoelectric sciFLEXARRAYER S1 microarray spotter and a piezo dispense capillary PDC 80 (Scienion AG, Berlin, Germany) [ ].

Techniques: Isolation, Labeling

Journal: Nutrients

Article Title: Significance of 1,25-Dihydroxyvitamin D 3 on Overall Mortality in Peritoneal Dialysis Patients with COVID-19

doi: 10.3390/nu15092050

Figure Lengend Snippet: Analysis of fibrinogen glycosylation performed using lectin-based glycoprotein microarray. Group 1—patients without calcitriol therapy, Group 2—patients on calcitriol therapy, p -statistical significance. All values are given as mean ± SD.

Article Snippet: Fibrinogen samples were printed on epoxysilane-coated microarray slides (NEXTERION Slide E, Schott, Germany) in eight identical subarrays using the piezoelectric printer sciFLEX-ARRAYER S1 equipped with the piezo dispensing capillary PDC 80 (Scienion AG, Berlin, Germany) at a temperature of 14 °C and humidity of 60%.

Techniques: Glycoproteomics, Microarray